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Using a reconstitution calculator Math mistakes account for a significant number of dosing errors

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Stoppers were removed, cells were washed twice and cultured in REBM supplemented with 0.5% FBS, 2.4 mM L-alanyl-L-glutamine and 1.8 mM CaCl 2 , with or without hHGF or dimeric macrocycle, and allowed to migrate into the migration zone

Nature Aging , 1, 760777

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discard if accidentally frozen Expiration monitoring Track reconstitution dates and discard after recommended timeframes Proper handling extends beyond storage